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ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18897-1-AP

Synonyms

EC:1.1.1.1, ADH3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH1C Polyclonal antibody (18897-1-AP)

    WB analysis using 18897-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18897-1-AP (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IP experiment of mouse liver using 18897-1-AP

    IP result of anti-ADH1C (IP:18897-1-AP, 4ug; Detection:18897-1-AP 1:1000) with mouse liver tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of rat liver using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human lung cancer using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human lung cancer using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human colon using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human colon using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ADH1C Polyclonal antibody (18897-1-AP)

    IHC staining of human kidney using 18897-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADH1C Polyclonal antibody (18897-1-AP)

    IF Staining of HepG2 using 18897-1-AP

    Immunofluorescent analysis of HepG2 cells, using ADH1C antibody 18897-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADH1C Polyclonal antibody (18897-1-AP)

    IF Staining of HepG2 using 18897-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1C antibody (18897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inrat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH1C fusion protein Ag13528
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 37-45 kDa
GenBank Accession NumberBC062476
Gene Symbol ADH1C
Gene ID (NCBI) 126
RRIDAB_10596950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

Protocols

Product Specific Protocols
WB protocol for ADH1C antibody 18897-1-APDownload protocol
IHC protocol for ADH1C antibody 18897-1-APDownload protocol
IF protocol for ADH1C antibody 18897-1-APDownload protocol
IP protocol for ADH1C antibody 18897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen
View Article
human,mouseWB

Biochem Pharmacol

The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis.

Authors - Feifeng Song
View Article
mouseWB

Biochem Pharmacol

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Authors - Yunhui Xu
View Article