ADH1C Polyclonal antibody
ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 18897-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | rat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ADH1C fusion protein Ag13528 |
Full Name | alcohol dehydrogenase 1C (class I), gamma polypeptide |
Calculated Molecular Weight | 375 aa, 40 kDa |
Observed Molecular Weight | 37-45 kDa |
GenBank Accession Number | BC062476 |
Gene Symbol | ADH1C |
Gene ID (NCBI) | 126 |
RRID | AB_10596950 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ADH1C antibody 18897-1-AP | Download protocol |
IHC protocol for ADH1C antibody 18897-1-AP | Download protocol |
IF protocol for ADH1C antibody 18897-1-AP | Download protocol |
IP protocol for ADH1C antibody 18897-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Biochem Pharmacol The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis. | ||
Biochem Pharmacol PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1. |