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Alpha Adducin Polyclonal antibody

Alpha Adducin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 10791-1-AP

Synonyms

ADD1, Alpha-adducin, adducin 1 (alpha), ADDA, ADD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using Alpha Adducin Polyclonal antibody (10791-1-AP)

    WB analysis of K-562 using 10791-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10791-1-AP (Alpha adducin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Alpha Adducin Polyclonal antibody (10791-1-AP)

    WB analysis of mouse testis using 10791-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10791-1-AP (Alpha adducin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Alpha Adducin Polyclonal antibody (10791-1-AP)

    IHC staining of human ovary cancer using 10791-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10791-1-AP (Alpha Adducin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Alpha Adducin Polyclonal antibody (10791-1-AP)

    IHC staining of human ovary cancer using 10791-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10791-1-AP (Alpha Adducin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Alpha Adducin Polyclonal antibody (10791-1-AP)

    IHC staining of human ovary tumor using 10791-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10791-1-AP (Alpha adducin antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Alpha Adducin Polyclonal antibody (10791-1-AP)

    IF Staining of mouse brain using 10791-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha Adducin antibody (10791-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10791-1-AP targets Alpha Adducin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Adducin fusion protein Ag1245
Full Name adducin 1 (alpha)
Calculated Molecular Weight 81 kDa, 84 kDa, 70 kDa, 73 kDa, 56 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC013393
Gene Symbol Alpha Adducin
Gene ID (NCBI) 118
RRIDAB_2257952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha Adducin antibody 10791-1-APDownload protocol
IHC protocol for Alpha Adducin antibody 10791-1-APDownload protocol
IF protocol for Alpha Adducin antibody 10791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Decoding the Synaptic Proteome with Long-Term Exposure to Midazolam during Early Development.

Authors - Nghi M Nguyen
View Article
bovineWB

Mol Biol Rep

Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells

Authors - Yue Wang
View Article