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ADAT2 Polyclonal antibody

ADAT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13621-1-AP

Synonyms

tRNA-specific adenosine deaminase 2, TAD2, EC:3.5.4.33, Deaminase domain-containing protein 1, DEADC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using ADAT2 Polyclonal antibody (13621-1-AP)

    WB analysis of A431 using 13621-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    WB analysis of mouse pancreas using 13621-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    WB analysis of mouse pancreas using 13621-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ADAT2 Polyclonal antibody (13621-1-AP)

    WB analysis of A431 using 13621-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    WB analysis of mouse brain using 13621-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    WB analysis of mouse brain using 13621-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IP experiment of mouse brain using 13621-1-AP

    IP result of anti-ADAT2 (IP:13621-1-AP, 4ug; Detection:13621-1-AP 1:300) with mouse brain tissue lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IHC staining of human colon cancer using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IHC staining of human colon cancer using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ADAT2 Polyclonal antibody (13621-1-AP)

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ADAT2 Polyclonal antibody (13621-1-AP)

    IF Staining of A431 using 13621-1-AP

    Immunofluorescent analysis of A431 cells using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13621-1-AP targets ADAT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAT2 fusion protein Ag4529
Full Name adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
Calculated Molecular Weight 191 aa, 21 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC037955
Gene Symbol ADAT2
Gene ID (NCBI) 134637
RRIDAB_10642142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ADAT2 antibody 13621-1-APDownload protocol
IHC protocol for ADAT2 antibody 13621-1-APDownload protocol
IF protocol for ADAT2 antibody 13621-1-APDownload protocol
IP protocol for ADAT2 antibody 13621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

The structure of the mouse ADAT2/ADAT3 complex reveals the molecular basis for mammalian tRNA wobble adenosine-to-inosine deamination.

Authors - Elizabeth Ramos-Morales
View Article