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ADAT1 Polyclonal antibody

ADAT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15482-1-AP

Synonyms

tRNA-specific adenosine-37 deaminase, tRNA-specific adenosine deaminase 1, hADAT1, EC:3.5.4.34, ADAT 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using ADAT1 Polyclonal antibody (15482-1-AP)

    WB analysis of mouse ovary using 15482-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15482-1-AP (ADAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using ADAT1 Polyclonal antibody (15482-1-AP)

    WB analysis of mouse ovary using 15482-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15482-1-AP (ADAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ADAT1 Polyclonal antibody (15482-1-AP)

    WB analysis of human heart using 15482-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15482-1-AP (ADAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ADAT1 Polyclonal antibody (15482-1-AP)

    IF Staining of MCF-7 using 15482-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 15482-1-AP (ADAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse ovary tissue, human heart tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15482-1-AP targets ADAT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAT1 fusion protein Ag7824
Full Name adenosine deaminase, tRNA-specific 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC002758
Gene Symbol ADAT1
Gene ID (NCBI) 23536
RRIDAB_2222792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADAT1 antibody 15482-1-APDownload protocol
IF protocol for ADAT1 antibody 15482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols