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ADAP1 Polyclonal antibody

ADAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13911-1-AP

Synonyms

Cnt a1, Centaurin-alpha-1, Centaurin alpha 1, CENTA1, ArfGAP with dual PH domains 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ADAP1 Polyclonal antibody (13911-1-AP)

    WB analysis of human brain using 13911-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ADAP1 Polyclonal antibody (13911-1-AP)

    WB analysis of human brain using 13911-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ADAP1 Polyclonal antibody (13911-1-AP)

    IHC staining of human gliomas using 13911-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ADAP1 Polyclonal antibody (13911-1-AP)

    IHC staining of human gliomas using 13911-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ADAP1 Polyclonal antibody (13911-1-AP)

    IF Staining of MCF-7 using 13911-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADAP1 Polyclonal antibody (13911-1-AP)

    IF Staining of HeLa using 13911-1-AP

    Immunofluorescent analysis of Hela cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13911-1-AP targets ADAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAP1 fusion protein Ag4898
Full Name ArfGAP with dual PH domains 1
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC033747
Gene Symbol ADAP1
Gene ID (NCBI) 11033
RRIDAB_2257889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADAP1 antibody 13911-1-APDownload protocol
IHC protocol for ADAP1 antibody 13911-1-APDownload protocol
IF protocol for ADAP1 antibody 13911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols