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ADAMTS1 Polyclonal antibody

ADAMTS1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12749-1-AP

Synonyms

ADAM-TS1, ADAM-TS 1, ADAMTS 1, ADAM TS1, ADAM TS 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADAMTS1 Polyclonal antibody (12749-1-AP)

    IHC staining of human liver cancer using 12749-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12749-1-AP (ADAMTS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADAMTS1 Polyclonal antibody (12749-1-AP)

    IHC staining of human liver cancer using 12749-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12749-1-AP (ADAMTS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADAMTS1 Polyclonal antibody (12749-1-AP)

    IF Staining of HepG2 using 12749-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12749-1-AP (ADAMTS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ADAMTS1 Polyclonal antibody (12749-1-AP)

    IF Staining of HEK-293 using 12749-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12749-1-AP (ADAMTS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12749-1-AP targets ADAMTS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAMTS1 fusion protein Ag3485
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 1
Calculated Molecular Weight 967 aa, 105 kDa
GenBank Accession NumberBC036515
Gene Symbol ADAMTS1
Gene ID (NCBI) 9510
RRIDAB_2877879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ADAMTS1 antibody 12749-1-APDownload protocol
IF protocol for ADAMTS1 antibody 12749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Front Aging Neurosci

Induction of A Disintegrin and Metalloproteinase with Thrombospondin motifs 1 by a rare variant or cognitive activities reduces hippocampal amyloid-β and consequent Alzheimer's disease risk

Authors - Yunjie Qiu
View Article
humanIHC

NPJ Precis Oncol

Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling

Authors - Shinji Kohsaka
View Article
mouseWB

Front Genet

Identification of polyunsaturated fatty acids related key modules and genes in metabolic dysfunction-associated fatty liver disease using WGCNA analysis

Authors - Cheng Xiao
View Article
ratWB

JOR Spine

Dynamics of N6-methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc

Authors - Libangxi Liu
View Article