ADAM5 Polyclonal antibody
ADAM5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 22147-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MCF-7 cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
22147-1-AP targets ADAM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ADAM5 fusion protein Ag17590 |
Full Name | ADAM metallopeptidase domain 5 pseudogene |
Calculated Molecular Weight | 412 aa, 47 kDa |
Observed Molecular Weight | 36-50 kDa |
GenBank Accession Number | BC067864 |
Gene Symbol | ADAM5/ADAM5P |
Gene ID (NCBI) | 255926 |
RRID | AB_11182171 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.
Protocols
Product Specific Protocols | |
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WB protocol for ADAM5 antibody 22147-1-AP | Download protocol |
IHC protocol for ADAM5 antibody 22147-1-AP | Download protocol |
IF protocol for ADAM5 antibody 22147-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |