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ADAM28 Polyclonal antibody
ADAM28 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 22234-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, BxPC-3 cells, mouse colon tissue, mouse liver tissue, mouse small intestine tissue, mouse spleen tissue, PC-13 cells |
| Positive IP detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
Product Information
The immunogen of 22234-1-AP is ADAM28 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADAM28 fusion protein Ag17586 |
| Full Name | ADAM metallopeptidase domain 28 |
| Calculated Molecular Weight | 775 aa, 87 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC136478 |
| Gene Symbol | ADAM28 |
| Gene ID (NCBI) | 10863 |
| RRID | AB_2879043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM28 antibody 22234-1-AP | Download protocol |
| IP protocol for ADAM28 antibody 22234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility | ||
Cancer Lett ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.
| ||
Biol Reprod ADGRG2 is dispensable for lumicrine signalling regulating epididymal initial segment differentiation and gene expression† | ||
Mol Cell Neurosci Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR. | ||
J Reprod Dev Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking testis-epididymis lumicrine signaling |
