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  • Featured Product
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ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14139-1-AP

Synonyms

MLTN, Meltrin-alpha, MCMP, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 12

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    WB analysis of mouse brain using 14139-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADAM12 Polyclonal antibody (14139-1-AP)

    WB analysis using 14139-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADAM12 Polyclonal antibody (14139-1-AP)

    WB analysis using 14139-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ADAM12 Polyclonal antibody (14139-1-AP)

    IP experiment of HeLa using 14139-1-AP

    IP result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human placenta using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human placenta using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human placenta using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human placenta using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human placenta using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human placenta using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human breast cancer using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human breast cancer using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human breast cancer using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ADAM12 Polyclonal antibody (14139-1-AP)

    IHC staining of human breast cancer using 14139-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADAM12 Polyclonal antibody (14139-1-AP)

    IF Staining of HepG2 using 14139-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADAM12 Polyclonal antibody (14139-1-AP)

    IF Staining of HepG2 using 14139-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse heart tissue, rat brain tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14139-1-AP targets ADAM12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM12 fusion protein Ag5206
Full Name ADAM metallopeptidase domain 12
Calculated Molecular Weight 100 kDa
Observed Molecular Weight 90-100 kDa, 70-80 kDa
GenBank Accession NumberBC060804
Gene Symbol ADAM12
Gene ID (NCBI) 8038
RRIDAB_2289230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Protocols

Product Specific Protocols
WB protocol for ADAM12 antibody 14139-1-APDownload protocol
IHC protocol for ADAM12 antibody 14139-1-APDownload protocol
IF protocol for ADAM12 antibody 14139-1-APDownload protocol
IP protocol for ADAM12 antibody 14139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIF

Cell Stem Cell

Vaccination-based immunotherapy to target profibrotic cells in liver and lung

Authors - Michal Sobecki
View Article
humanIHC

J Cell Biol

Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.

Authors - Díaz Bego?a B
View Article
humanIF

Theranostics

Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Authors - Yinan Chen
View Article
humanWB,IF

Cell Death Differ

ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.

Authors - M Aghababaei
View Article
humanIHC

J Hematol Oncol

Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.

Authors - Kosuke Ogawa
View Article
humanWB,IHC

Oncogene

A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion.

Authors - Rao V H VH
View Article