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ACY3 Polyclonal antibody

ACY3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16786-1-AP

Synonyms

Aspartoacylase-2, ASPA2, Aminoacylase-3, Acylase III, ACY-3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ACY3 Polyclonal antibody (16786-1-AP)

    WB analysis of mouse liver using 16786-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16786-1-AP (ACY3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ACY3 Polyclonal antibody (16786-1-AP)

    WB analysis of mouse liver using 16786-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16786-1-AP (ACY3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ACY3 Polyclonal antibody (16786-1-AP)

    IHC staining of human colon using 16786-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16786-1-AP (ACY3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16786-1-AP targets ACY3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACY3 fusion protein Ag10220
Full Name aspartoacylase (aminocyclase) 3
Calculated Molecular Weight 319 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC008689
Gene Symbol ACY3
Gene ID (NCBI) 91703
RRIDAB_2222751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACY3 antibody 16786-1-APDownload protocol
IHC protocol for ACY3 antibody 16786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Comparison of spinocerebellar ataxia type 3 mouse models identifies early gain-of-function, cell-autonomous transcriptional changes in oligodendrocytes.

Authors - Biswarathan Ramani
View Article