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ACTR1B Polyclonal antibody

ACTR1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11023-1-AP

Synonyms

Beta-centractin, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTR1B Polyclonal antibody (11023-1-AP)

    WB analysis using 11023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11023-1-AP (ACTR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTR1B Polyclonal antibody (11023-1-AP)

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTR1B Polyclonal antibody (11023-1-AP)

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ACTR1B Polyclonal antibody (11023-1-AP)

    IF Staining of MCF-7 using 11023-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ACTR1B antibody 11023-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue, mouse heart
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:300
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11023-1-AP targets ACTR1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTR1B fusion protein Ag1467
Full Name ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC010090
Gene Symbol ACTR1B
Gene ID (NCBI) 10120
RRIDAB_2877742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTR1B (also known as beta centractin) is a part of the 20S dynactin complex. It colocalizes with centrosomes and vesicular (membranous) structures and is involved in cell division. ACTR1B and ACTR1A share 90% amino acid identity, and this antibody recognizes both ACTR1B and ACTR1A.

Protocols

Product Specific Protocols
WB protocol for ACTR1B antibody 11023-1-APDownload protocol
IHC protocol for ACTR1B antibody 11023-1-APDownload protocol
IF protocol for ACTR1B antibody 11023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols