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  • Featured Product
  • KD/KO Validated

ACTN4 Polyclonal antibody

ACTN4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19096-1-AP

Synonyms

Alpha actinin 4, Alpha-actinin-4, ACTN 4, actinin, alpha 4, Actinin alpha 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTN4 Polyclonal antibody (19096-1-AP)

    WB analysis using 19096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19096-1-AP (ACTN4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    WB analysis of mouse liver using 19096-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19096-1-AP (ACTN4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ACTN4 Polyclonal antibody (19096-1-AP)

    IP experiment of HeLa using 19096-1-AP

    IP result of anti-ACTN4 (IP:19096-1-AP, 5ug; Detection:19096-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    IHC staining of human lung cancer using 19096-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    IHC staining of human colon using 19096-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    IHC staining of human breast cancer using 19096-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    IHC staining of human breast cancer using 19096-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19096-1-AP (ACTN4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    IHC staining of human lung cancer using 19096-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 19096-1-AP (ACTN4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACTN4 Polyclonal antibody (19096-1-AP)

    IHC staining of human lung cancer using 19096-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 19096-1-AP (ACTN4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ACTN4 Polyclonal antibody (19096-1-AP)

    IF Staining of MCF-7 using 19096-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ACTN4 antibody (19096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ACTN4 Polyclonal antibody (19096-1-AP)

    IF Staining of HeLa using 19096-1-AP

    Immunofluorescent analysis of Hela cells, using ACTN4 antibody 19096-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ACTN4 Polyclonal antibody (19096-1-AP)

    IF Staining of MCF-7 using 19096-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19096-1-AP (ACTN4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, mouse liver tissue, HeLa cells, Jurkat cells, C2C12 cells, NIH/3T3 cells, mouse skeletal muscle, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19096-1-AP targets ACTN4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN4 fusion protein Ag13546
Full Name actinin, alpha 4
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC005033
Gene Symbol ACTN4
Gene ID (NCBI) 81
RRIDAB_10642150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Protocols

Product Specific Protocols
WB protocol for ACTN4 antibody 19096-1-APDownload protocol
IHC protocol for ACTN4 antibody 19096-1-APDownload protocol
IF protocol for ACTN4 antibody 19096-1-APDownload protocol
IP protocol for ACTN4 antibody 19096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanELISA

J Thorac Oncol

Alpha-actinin 4 is Associated with Cancer Cell Motility and is a Potential Biomarker in Non-Small-Cell Lung Cancer.

Authors - Ming-Chuan Wang
View Article
humanWB,IHC

Oncogene

KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma

Authors - Zhenyu Yang
View Article
humanWB

Oncogene

MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

Authors - Agarwal N N
View Article
humanWB

Cancer Immunol Immunother

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Authors - Xiuxing Chen
View Article
ratWB,IF

Sci Rep

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Authors - Hongfei Ge
View Article
mouseIF

J Cell Sci

GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region

Authors - Paroma Chatterjee
View Article