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ACTN3 Polyclonal antibody

ACTN3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24378-1-AP

Synonyms

actinin, alpha 3, ACTN3, Alpha actinin 3, F actin cross linking protein

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    WB analysis of mouse skeletal muscle using 24378-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24378-1-AP (ACTN3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    IHC staining of mouse skeletal muscle using 24378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    IHC staining of mouse skeletal muscle using 24378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    IHC staining of human breast cancer using 24378-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    IHC staining of human breast cancer using 24378-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    IF Staining of mouse skeletal muscle using 24378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ACTN3 Polyclonal antibody (24378-1-AP)

    IF Staining of mouse skeletal muscle using 24378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24378-1-AP targets ACTN3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN3 fusion protein Ag19479
Full Name actinin, alpha 3
Calculated Molecular Weight 901 aa, 103 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC099647
Gene Symbol ACTN3
Gene ID (NCBI) 89
RRIDAB_2879515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACTN3 antibody 24378-1-APDownload protocol
IHC protocol for ACTN3 antibody 24378-1-APDownload protocol
IF protocol for ACTN3 antibody 24378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Dokl Biochem Biophys

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Authors - I O Petrova
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Joleen (Verified Customer) (06-12-2019)

The antibody recognizes ACTN3 (top band) but also other non-specific proteins, though less prominently.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ACTN3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:24378-1-AP)