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ACTN2 Polyclonal antibody

ACTN2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 14221-1-AP

Synonyms

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, Alpha actinin 2, ACTN 2, actinin, alpha 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTN2 Polyclonal antibody (14221-1-AP)

    WB analysis using 14221-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    WB analysis of mouse lung using 14221-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    WB analysis of mouse skeletal muscle using 14221-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C2C12 cells using ACTN2 Polyclonal antibody (14221-1-AP)

    WB analysis of C2C12 using 14221-1-AP

    C2C12 cell were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ACTN2 Polyclonal antibody (14221-1-AP)

    IP experiment of HeLa using 14221-1-AP

    IP result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    IHC staining of mouse heart using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    IHC staining of mouse heart using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    IHC staining of mouse skeletal muscle using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    IHC staining of mouse skeletal muscle using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    IF Staining of mouse heart using 14221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ACTN2 Polyclonal antibody (14221-1-AP)

    IF Staining of mouse heart using 14221-1-AP

    Mouse cardiac tissue slice of 300µm thickness labelled with alpha actinin (14221-1-AP). Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.

  • Immunofluorescence (IF) / fluorescent staining of Human iPSC derived cardiomyocyte using ACTN2 Polyclonal antibody (14221-1-AP)

    IF Staining of Human iPSC derived cardiomyocyte using 14221-1-AP

    Human iPSC derived cardiomyocyte in 2D culture, with alpha actinin (14221-1-AP) and DAPI. Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ACTN2 Polyclonal antibody (14221-1-AP)

    IF Staining of C2C12 using 14221-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ACTN2 antibody (14221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ACTN2 Polyclonal antibody (14221-1-AP)

    IF Staining of NIH/3T3 using 14221-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ACTN2 Polyclonal antibody (14221-1-AP)

    IF Staining of C2C12 using 14221-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using 14221-1-AP (ACTN2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse heart, mouse kidney, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells, NIH/3T3 cells, Human iPSC derived cardiomyocyte

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14221-1-AP targets ACTN2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN2 fusion protein Ag5459
Full Name actinin, alpha 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC051770
Gene Symbol ACTN2
Gene ID (NCBI) 88
RRIDAB_2221547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

Protocols

Product Specific Protocols
WB protocol for ACTN2 antibody 14221-1-APDownload protocol
IHC protocol for ACTN2 antibody 14221-1-APDownload protocol
IF protocol for ACTN2 antibody 14221-1-APDownload protocol
IP protocol for ACTN2 antibody 14221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Adv Mater

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring

Authors - Huihui He
View Article
mouseWB,IF

Nat Commun

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Authors - Weiyi Qian
View Article
ratWB

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
View Article
humanIF

Dev Cell

Essential role of MESP1-RING1A complex in cardiac differentiation

Authors - Qianqian Liang
View Article
humanIF

Front Cell Dev Biol

Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy.

Authors - Bin Li
View Article
humanWB,IF

Front Cell Dev Biol

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Authors - Guillaume Gilbert
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Rebecca (Verified Customer) (09-21-2022)

20ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: iPSC-CM
ACTN2 Antibody Western Blot validation (1:10000 dilution) in iPSC-CM (Cat no:14221-1-AP)