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ACTL7B Polyclonal antibody

ACTL7B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13537-1-AP

Synonyms

Actin like 7 beta, actin like 7B, Actin like protein 7B, ACTL7B

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    WB analysis of human testis using 13537-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    WB analysis of mouse testis using 13537-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    WB analysis of human liver using 13537-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    IHC staining of human prostate cancer using 13537-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACTL7B Polyclonal antibody (13537-1-AP)

    IHC staining of human prostate cancer using 13537-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman testis tissue, human liver tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13537-1-AP targets ACTL7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTL7B fusion protein Ag4460
Full Name actin-like 7B
Calculated Molecular Weight 415 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC033789
Gene Symbol ACTL7B
Gene ID (NCBI) 10880
RRIDAB_2223776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTL7B is also named as actin-like-7-beta. The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. The ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. Diseases associated with ACTL7B include Dysautonomia and Hypertensive Nephropathy. An important paralog of this gene is ACTL7A. ACTL7B can be detected as 45 kDa.

Protocols

Product Specific Protocols
WB protocol for ACTL7B antibody 13537-1-APDownload protocol
IHC protocol for ACTL7B antibody 13537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Rep

The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.

Authors - Brian P Hermann
View Article
human,mouseWB

Development

Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis

Authors - Gina E Merges
View Article