beta Actin Antibody 53 Publications

Rabbit Polyclonal| Catalog number: 20536-1-AP

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Size: 09µ/gram

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Species specificity:
Human,Mouse,Rat,Zebrafish, Monkey

Positive WB detected in:
HeLa cells, A431 cells, A549 cells, C6 cells, HEK-293 cells, HepG2 cells, human testis tissue, Jurkat cells, MCF7 cells, mouse brain tissue, mouse lung tissue, mouse spleen tissue, mouse uterus tissue, NIH/3T3 cells, rat brain tissue, SGC-7901 cells, Sp2/0 cells

Positive IHC detected in:
human skeletal muscle tissue, human colon tissue, human heart tissue, human kidney tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:1000-1:10000
IHC : 1:20-1:500
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

Immunogen Information


Full name:
actin, beta

Calculated molecular weight:
375aa,42 kDa

Observed molecular weight:
42 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ACTB

Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.


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