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smooth muscle actin specific Polyclonal antibody

smooth muscle actin specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55135-1-AP

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 Smooth muscle Actin Specific, Actin, aortic smooth muscle, Alpha actin 2, Alpha SMA, Alpha SMA Specific, a-SMA, smooth muscle actin, smooth muscle actin-specific, α SMA, α-sma

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    WB analysis using 55135-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    WB analysis using 55135-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    WB analysis of mouse kidney using 55135-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human heart using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human heart using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human liver cancer using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human liver cancer using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of mouse heart using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of rat colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of rat colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human heart using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human colon cancer using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human colon cancer using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of mouse liver using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of mouse liver using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human heart using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of mouse colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of mouse colon using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human hepatocirrhosis using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IHC staining of human hepatocirrhosis using 55135-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IF Staining of mouse heart using 55135-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using smooth muscle actin specific Polyclonal antibody (55135-1-AP)

    IF Staining of mouse heart using 55135-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung
Positive IHC detected inmouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55135-1-AP targets smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001613
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_10949628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

Protocols

Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 55135-1-APDownload protocol
IHC protocol for smooth muscle actin specific antibody 55135-1-APDownload protocol
IF protocol for smooth muscle actin specific antibody 55135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

ACS Nano

Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction

Authors - Xiaoqian Ji
View Article
humanIHC

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
View Article
mouseIF

Nat Commun

Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis.

Authors - Wen Gao
View Article
humanIF

Mol Cancer

Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts.

Authors - Jing Kong
View Article
mouseWB,IF

J Nanobiotechnology

Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy

Authors - Zhenxian Li
View Article
human,mouseWB,IF

Aging Dis

HIF-1α promotes the migration and invasion of cancer-associated fibroblasts by miR-210

Authors - Ying Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

S (Verified Customer) (09-23-2024)

Excelllent

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: A549
smooth muscle actin specific Antibody Western Blot validation (1000 dilution) in A549 (Cat no:55135-1-AP)
FH

PK (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: cardiac Fibroblast
smooth muscle actin specific Antibody Western Blot validation ( dilution) in cardiac Fibroblast (Cat no:55135-1-AP)
FH

S (Verified Customer) (12-01-2021)

Excellent

  • Applications: Western Blot
  • Cell Tissue Type: LUNG FIBROBLASTS
smooth muscle actin specific Antibody Western Blot validation ( dilution) in LUNG FIBROBLASTS (Cat no:55135-1-AP)