ACTA1-Specific Polyclonal antibody
ACTA1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), ELISA, IF
Conjugate
Unconjugated
Cat no : 17521-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 3 publications below |
Product Information
17521-1-AP targets ACTA1-Specific in WB, IHC, FC (Intra), ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | actin, alpha 1, skeletal muscle |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39-42 kDa |
| GenBank Accession Number | NM_001100 |
| Gene Symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_2273608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| IHC protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| FC protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome. | ||
Cancer Lett Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop. | ||
Mol Ther Nucleic Acids Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis | ||
Int J Mol Sci Neddylation Regulates Class IIa and III Histone Deacetylases to Mediate Myoblast Differentiation | ||
Aging (Albany NY) Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats. | ||
iScience A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart |
