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ACSS3 Polyclonal antibody

ACSS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16204-1-AP

Synonyms

EC:6.2.1.17, EC:6.2.1.1, Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS3 Polyclonal antibody (16204-1-AP)

    WB analysis using 16204-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16204-1-AP (ACSS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACSS3 Polyclonal antibody (16204-1-AP)

    WB analysis of HepG2 using 16204-1-AP

    WB result of ACSS3 antibody (16204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSS3 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACSS3 Polyclonal antibody (16204-1-AP)

    IHC staining of human liver using 16204-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16204-1-AP (ACSS3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACSS3 Polyclonal antibody (16204-1-AP)

    IHC staining of human liver using 16204-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16204-1-AP (ACSS3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ACSS3 Polyclonal antibody (16204-1-AP)

    IF Staining of U2OS using 16204-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ACSS3 antibody (16204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ACSS3 Polyclonal antibody (16204-1-AP)

    IF Staining of U2OS using 16204-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ACSS3 antibody (16204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16204-1-AP targets ACSS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSS3 fusion protein Ag9173
Full Name acyl-CoA synthetase short-chain family member 3
Calculated Molecular Weight 686 aa, 75 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC009317
Gene Symbol ACSS3
Gene ID (NCBI) 79611
RRIDAB_2257893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSS3(acyl-CoA synthetase short-chain family member 3, mitochondrial) belongs to the ATP-dependent AMP-binding enzyme family. It activates acetate so that it can be used for lipid synthesis or for energy generation. The deduced 686-amino acid protein contains 4 of 5 motifs characteristic of acyl-CoA synthetases. This protein has 2 isoforms produced by alternative splicing. The full length protein has a transit peptide with 29 amino acids.

Protocols

Product Specific Protocols
WB protocol for ACSS3 antibody 16204-1-APDownload protocol
IHC protocol for ACSS3 antibody 16204-1-APDownload protocol
IF protocol for ACSS3 antibody 16204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Metab

Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11

Authors - Bing Zhou
View Article
human,mouseWB,IHC

Cancer Commun (Lond)

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

Authors - Xiaoteng Cui
View Article
mouseWB

Nutrients

Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.

Authors - Karolin Weitkunat
View Article
human,mouseWB

Biochem J

NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on esophageal squamous cell carcinoma.

Authors - Joab Otieno Odera
View Article
mouseWB

Food Chem Toxicol

Integration of proteomics and network toxicology reveals the mechanism of mercury chloride induced hepatotoxicity, in mice and HepG2 cells

Authors - Xin Cao
View Article
humanIHC,WB

Biochim Biophys Acta Mol Basis Dis

ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis

Authors - Lan Wang
View Article