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  • KD/KO Validated

ACSS1 Polyclonal antibody

ACSS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 17138-1-AP

Synonyms

ACAS2L, ACECS1, AceCS2L, Acetyl coenzyme A synthetase 2 like, mitochondrial, ACSS1, KIAA1846



Tested Applications

Positive WB detected inCaco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17138-1-AP targets ACSS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSS1 fusion protein Ag10896
Full Name acyl-CoA synthetase short-chain family member 1
Calculated Molecular Weight 689 aa, 75 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC039261
Gene Symbol ACSS1
Gene ID (NCBI) 84532
RRIDAB_2289182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.

Protocols

Product Specific Protocols
WB protocol for ACSS1 antibody 17138-1-APDownload protocol
IHC protocol for ACSS1 antibody 17138-1-APDownload protocol
IP protocol for ACSS1 antibody 17138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
  • KD Validated
humanWB,IHC

Nat Commun

PMID: 27357947

Authors - Xue Gao
humanWB

EMBO J

Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice.

Authors - Judith Schweisgut
pigIF,WB

J Cell Physiol

Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.

Authors - Wenjun Zhou
  • KO Validated
humanWB, IHC

PLoS Comput Biol

Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples.

Authors - Xiaoqiang Sun
ratWB

Front Pharmacol

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Authors - Han-Qing Pang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Marina (Verified Customer) (12-18-2020)

I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Macrophages
ACSS1 Antibody Western Blot validation (1:500 dilution) in Macrophages (Cat no:17138-1-AP)
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain