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ACSM3 Polyclonal antibody

ACSM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10168-2-AP

Synonyms

EC:6.2.1.17, Butyryl-coenzyme A synthetase 3, Butyrate--CoA ligase 3, Acyl-coenzyme A synthetase ACSM3, mitochondrial, Acyl-CoA synthetase medium-chain family member 3



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10168-2-AP targets ACSM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSM3 fusion protein Ag0224
Full Name acyl-CoA synthetase medium-chain family member 3
Calculated Molecular Weight 49 kDa, 66 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC002790
Gene Symbol ACSM3
Gene ID (NCBI) 6296
RRIDAB_2222699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.

Protocols

Product Specific Protocols
WB protocol for ACSM3 antibody 10168-2-APDownload protocol
IHC protocol for ACSM3 antibody 10168-2-APDownload protocol
IF protocol for ACSM3 antibody 10168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIHC

Cell

Organoid Models of Human and Mouse Ductal Pancreatic Cancer.

Authors - Sylvia F Boj
humanIHC

Front Cell Dev Biol

Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.

Authors - Lingyun Zhang
humanWB, IHC

Front Oncol

The Overexpression of Acyl-CoA Medium-Chain Synthetase-3 (ACSM3) Suppresses the Ovarian Cancer Progression via the Inhibition of Integrin β1/AKT Signaling Pathway.

Authors - Limei Yan
mouse

Sci Rep

Thioesterase induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation.

Authors - Giovan N Cholico
mouseWB,IF

Eur J Cell Biol

Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy.

Authors - Paul Dowling
humanWB

Exp Ther Med

ACSM3 suppresses proliferation and induces apoptosis and cell cycle arrest in acute myeloid leukemia cells via the regulation of IGF2BP2

Authors - Xin Zheng