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ACSM2A Polyclonal antibody

ACSM2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22862-1-AP

Synonyms

A 923A4.1, ACSM2, ACSM2A, Butyrate CoA ligase 2A, MACS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSM2A Polyclonal antibody (22862-1-AP)

    WB analysis using 22862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ACSM2A Polyclonal antibody (22862-1-AP)

    WB analysis of human brain using 22862-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM2A Polyclonal antibody (22862-1-AP)

    IHC staining of human liver cancer using 22862-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM2A Polyclonal antibody (22862-1-AP)

    IHC staining of human liver cancer using 22862-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACSM2A Polyclonal antibody (22862-1-AP)

    IF Staining of HepG2 using 22862-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22862-1-AP (ACSM2A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue, rat liver tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22862-1-AP is ACSM2A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSM2A fusion protein Ag18856
Full Name acyl-CoA synthetase medium-chain family member 2A
Calculated Molecular Weight 577 aa, 64 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberBC125176
Gene Symbol ACSM2A
Gene ID (NCBI) 123876
RRIDAB_2879181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSM2A(Acyl-coenzyme A synthetase ACSM2A, mitochondrial) is also named as ACSM2, MACS2 and belongs to the ATP-dependent AMP-binding enzyme family. It acts as a medium-chain fatty acid : CoA ligase with the caculated molecular weight of 64 kDa and acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids.

Protocols

Product Specific Protocols
WB protocol for ACSM2A antibody 22862-1-APDownload protocol
IHC protocol for ACSM2A antibody 22862-1-APDownload protocol
IF protocol for ACSM2A antibody 22862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Physiol

Proteomics analyses of acute kidney injury biomarkers in a rat exertional heat stroke model

Authors - Fu-Li Wen
View Article
mouseWB

Front Mol Med

Loss of PPARα function promotes epigenetic dysregulation of lipid homeostasis driving ferroptosis and pyroptosis lipotoxicity in metabolic dysfunction associated Steatotic liver disease (MASLD)

Authors - Claudia Theys
View Article