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ACSL3 Polyclonal antibody

ACSL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20710-1-AP

Synonyms

EC:6.2.1.3, EC:6.2.1.2, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 3



Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HuH-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20710-1-AP targets ACSL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name acyl-CoA synthetase long-chain family member 3
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberNM_004457
Gene Symbol ACSL3
Gene ID (NCBI) 2181
RRIDAB_10697670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

Protocols

Product Specific Protocols
WB protocol for ACSL3 antibody 20710-1-APDownload protocol
IHC protocol for ACSL3 antibody 20710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Commun (Lond)

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

Authors - Xiaoteng Cui
humanWB

Clin Transl Med

NAT10: An RNA cytidine transferase regulates fatty acid metabolism in cancer cells

Authors - Mahmood Hassan Dalhat
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu

Sci Rep

Morphologically and Functionally Distinct Lipid Droplet Subpopulations.

Authors - Shuyan Zhang
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Rab18 binds PLIN2 and ACSL3 to mediate lipid droplet dynamics.

Authors - Yaqin Deng
humanWB

J Cell Sci

Exploring the ATG9A interactome uncovers interaction with VPS13A

Authors - Alexander R van Vliet