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ACSL1 Polyclonal antibody

ACSL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13989-1-AP

Synonyms

EC:6.2.1.24, EC:6.2.1.15, Arachidonate--CoA ligase, Acyl-CoA synthetase 1, Acyl CoA synthetase 1



Tested Applications

Positive WB detected inL02 cells, mouse cerebellum tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13989-1-AP targets ACSL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL1 fusion protein Ag5059
Full Name acyl-CoA synthetase long-chain family member 1
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 68 kDa, 78 kDa
GenBank Accession NumberBC050073
Gene Symbol ACSL1
Gene ID (NCBI) 2180
RRIDAB_2257870
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSL1 antibody 13989-1-APDownload protocol
IHC protocol for ACSL1 antibody 13989-1-APDownload protocol
IF protocol for ACSL1 antibody 13989-1-APDownload protocol
IP protocol for ACSL1 antibody 13989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
mouseIF

Nat Cell Biol

Functional multi-organelle units control inflammatory lipid metabolism of macrophages

Authors - Julia A Zimmermann
humanWB

Hepatology

Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.

Authors - Ming-Da Wang
humanIF

Theranostics

Mesenchymal glioblastoma constitutes a major ceRNA signature in the TGF-β pathway.

Authors - Qixue Wang
humanWB, IHC

Cancer Res

Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.

Authors - Ming Cui
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Thomas (Verified Customer) (10-23-2019)

Very good service, very good antibody

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Rattus heart