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ACSL1 Polyclonal antibody
ACSL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13989-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, mouse cerebellum tissue, mouse liver tissue, rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 45 publications below |
IHC | See 7 publications below |
IF | See 3 publications below |
IP | See 2 publications below |
Product Information
13989-1-AP targets ACSL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACSL1 fusion protein Ag5059 |
Full Name | acyl-CoA synthetase long-chain family member 1 |
Calculated Molecular Weight | 78 kDa |
Observed Molecular Weight | 68 kDa, 78 kDa |
GenBank Accession Number | BC050073 |
Gene Symbol | ACSL1 |
Gene ID (NCBI) | 2180 |
RRID | AB_2257870 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for ACSL1 antibody 13989-1-AP | Download protocol |
IHC protocol for ACSL1 antibody 13989-1-AP | Download protocol |
IF protocol for ACSL1 antibody 13989-1-AP | Download protocol |
IP protocol for ACSL1 antibody 13989-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Functional multi-organelle units control inflammatory lipid metabolism of macrophages | ||
Hepatology Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients. | ||
Theranostics Mesenchymal glioblastoma constitutes a major ceRNA signature in the TGF-β pathway. | ||
Cancer Res Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway. | ||
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Thomas (Verified Customer) (10-23-2019) | Very good service, very good antibody
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