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  • KD/KO Validated

ACSF2 Polyclonal antibody

ACSF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16140-1-AP

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2



Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16140-1-AP targets ACSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSF2 fusion protein Ag9199
Full Name acyl-CoA synthetase family member 2
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC012053
Gene Symbol ACSF2
Gene ID (NCBI) 80221
RRIDAB_2878222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

Protocols

Product Specific Protocols
WB protocol for ACSF2 antibody 16140-1-APDownload protocol
IHC protocol for ACSF2 antibody 16140-1-APDownload protocol
IF protocol for ACSF2 antibody 16140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IP

Prostaglandins Other Lipid Mediat

Targeted Lipidomics Analysis of Lysine 179 Acetylation of ACSF2 in Rat Hepatic Stellate Cells

Authors - Meili Wang
mouse,ratWB,IHC

Life Sci

ACSF2-mediated ferroptosis is involved in ulcerative colitis

Authors - Lianxiang Luo
humanWB,IF

Free Radic Biol Med

Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells

Authors - Haoyu Shi
  • KD Validated
humanIHC

Clin Cancer Res

Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics

Authors - Xiaoqi Peng
human,mouseWB,IHC,IF

Diabetologia

ACSF2 and lysine lactylation contribute to renal tubule injury in diabetes

Authors - Jingfang Chen
humanWB,CoIP

Mol Carcinog

M2 macrophage exosome-derived Apoc1 promotes ferroptosis resistance in osteosarcoma by inhibiting ACSF2 deubiquitination

Authors - Ping Yin