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ACPP Monoclonal antibody

ACPP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G2C9

Cat no : 60176-1-Ig

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ACPP Monoclonal antibody (60176-1-Ig)

    WB analysis of K-562 using 60176-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ACPP Monoclonal antibody (60176-1-Ig)

    WB analysis of human testis using 60176-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, human testis tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ACPP fusion protein Ag8578
Full Name acid phosphatase, prostate
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC007460
Gene Symbol ACPP
Gene ID (NCBI) 55
RRIDAB_10733336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Protocols

Product Specific Protocols
WB protocol for ACPP antibody 60176-1-IgDownload protocol
IHC protocol for ACPP antibody 60176-1-IgDownload protocol
IF protocol for ACPP antibody 60176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Endocrinol (Lausanne)

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.

Authors - Chong Wang
View Article