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ACP1 Polyclonal antibody

ACP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22214-1-AP

Synonyms

acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ACP1 Polyclonal antibody (22214-1-AP)

    WB analysis of mouse brain using 22214-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ACP1 Polyclonal antibody (22214-1-AP)

    WB analysis of mouse brain using 22214-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using ACP1 Polyclonal antibody (22214-1-AP)

    WB analysis of 4T1 using 22214-1-AP

    4T1 cells were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACP1 Polyclonal antibody (22214-1-AP)

    IHC staining of human breast cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACP1 Polyclonal antibody (22214-1-AP)

    IHC staining of human breast cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACP1 Polyclonal antibody (22214-1-AP)

    IHC staining of human lung cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACP1 Polyclonal antibody (22214-1-AP)

    IHC staining of human lung cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22214-1-AP targets ACP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACP1 fusion protein Ag17539
Full Name acid phosphatase 1, soluble
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC007422
Gene Symbol ACP1
Gene ID (NCBI) 52
RRIDAB_2879032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human red cell acid phosphatase (ACP1) is a polymorphic enzyme closely related to cytosolic low molecular weight acid phosphatases, a protein family broadly conserved among eukaryotes. It catalyses the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACP1 antibody 22214-1-APDownload protocol
IHC protocol for ACP1 antibody 22214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang
View Article