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ACOX2 Polyclonal antibody

ACOX2 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17571-1-AP

Synonyms

Trihydroxycoprostanoyl-CoA oxidase, THCA-CoA oxidase, Peroxisomal acyl-coenzyme A oxidase 2, EC:1.17.99.3, 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX2 Polyclonal antibody (17571-1-AP)

    WB analysis using 17571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17571-1-AP (ACOX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ACOX2 Polyclonal antibody (17571-1-AP)

    IP experiment of mouse liver using 17571-1-AP

    IP result of anti-ACOX2 (IP:17571-1-AP, 4ug; Detection:17571-1-AP 1:300) with mouse liver tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACOX2 Polyclonal antibody (17571-1-AP)

    IHC staining of human liver using 17571-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17571-1-AP (ACOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACOX2 Polyclonal antibody (17571-1-AP)

    IHC staining of human liver using 17571-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17571-1-AP (ACOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACOX2 Polyclonal antibody (17571-1-AP)

    IF Staining of HepG2 using 17571-1-AP

    Immunofluorescent analysis of HepG2 cells, using ACOX2 antibody 17571-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tisue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17571-1-AP targets ACOX2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOX2 fusion protein Ag11778
Full Name acyl-Coenzyme A oxidase 2, branched chain
Calculated Molecular Weight 681 aa, 77 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC047700
Gene Symbol ACOX2
Gene ID (NCBI) 8309
RRIDAB_2221803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACOX2 antibody 17571-1-APDownload protocol
IHC protocol for ACOX2 antibody 17571-1-APDownload protocol
IF protocol for ACOX2 antibody 17571-1-APDownload protocol
IP protocol for ACOX2 antibody 17571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Hypertens Res

Increased expression of acyl-CoA oxidase 2 in the kidney with plasma phytanic acid and altered gut microbiota in spontaneously hypertensive rats.

Authors - Masahiro Okamura
View Article