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  • Featured Product
  • KD/KO Validated

ACOT9 Polyclonal antibody

ACOT9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15901-1-AP

Synonyms

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOT9 Polyclonal antibody (15901-1-AP)

    WB analysis using 15901-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACOT9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ACOT9 Polyclonal antibody (15901-1-AP)

    WB analysis of MCF-7 using 15901-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ACOT9 Polyclonal antibody (15901-1-AP)

    WB analysis of MCF-7 using 15901-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    WB analysis of mouse heart using 15901-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of mouse kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of mouse kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of mouse kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of mouse kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of rat kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of rat kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT9 Polyclonal antibody (15901-1-AP)

    IHC staining of human kidney using 15901-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15901-1-AP targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT9 fusion protein Ag8730
Full Name acyl-CoA thioesterase 9
Calculated Molecular Weight 24 kDa, 49 kDa
Observed Molecular Weight 43-50 kDa
GenBank Accession NumberBC012573
Gene Symbol ACOT9
Gene ID (NCBI) 23597
RRIDAB_2221519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

Protocols

Product Specific Protocols
WB protocol for ACOT9 antibody 15901-1-APDownload protocol
IHC protocol for ACOT9 antibody 15901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Liver Int

Acyl-CoA thioesterase 9 promotes tumor growth and metastasis through reprogramming of fatty acid metabolism in hepatocellular carcinoma

Authors - Bao Wang
  • KD Validated
View Article
mouseWB

EMBO Mol Med

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy

Authors - Nneka Southwell
View Article