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ACOT2 Polyclonal antibody

ACOT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15633-1-AP

Synonyms

EC:3.1.2.2, CTE-Ia, Acyl-coenzyme A thioesterase 2, mitochondrial, Acyl-coenzyme A thioester hydrolase 2a, Acyl-CoA thioesterase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis using 15633-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis of mouse kidney using 15633-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis of HepG2 using 15633-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis of human testis using 15633-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis of HEK-293 using 15633-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis of human brain using 15633-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    WB analysis of human kidney using 15633-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ACOT2 Polyclonal antibody (15633-1-AP)

    IP experiment of HepG2 using 15633-1-AP

    IP result of anti-ACOT2 (IP:15633-1-AP, 3ug; Detection:15633-1-AP 1:500) with HepG2 cells lysate 1700ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human heart using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human heart using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human kidney using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15633-1-AP (ACOT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human kidney using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15633-1-AP (ACOT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human skeletal muscle using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human skeletal muscle using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human kidney using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human kidney using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human testis using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human testis using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human liver using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human liver using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human spleen using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human spleen using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human ovary using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ACOT2 Polyclonal antibody (15633-1-AP)

    IHC staining of human ovary using 15633-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACOT2 Polyclonal antibody (15633-1-AP)

    IF Staining of HepG2 using 15633-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT2 antibody (15633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACOT2 Polyclonal antibody (15633-1-AP)

    IF Staining of HepG2 using 15633-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT2 antibody (15633-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HepG2 cells, human testis tissue, human brain tissue, human kidney tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue, human heart tissue, human testis tissue, human liver tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15633-1-AP targets ACOT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT2 fusion protein Ag8093
Full Name acyl-CoA thioesterase 2
Calculated Molecular Weight 483 aa, 53 kDa
Observed Molecular Weight 46-53 kDa
GenBank Accession NumberBC006335
Gene Symbol ACOT2
Gene ID (NCBI) 10965
RRIDAB_2221535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300).

Protocols

Product Specific Protocols
WB protocol for ACOT2 antibody 15633-1-APDownload protocol
IHC protocol for ACOT2 antibody 15633-1-APDownload protocol
IF protocol for ACOT2 antibody 15633-1-APDownload protocol
IP protocol for ACOT2 antibody 15633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi
View Article
mouseWB

Life Sci Alliance

High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo

Authors - Nina A Bonekamp
View Article
mouseWB

Biomed Res Int

Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression.

Authors - Yuka Murata
View Article
mouseWB

J Biol Chem

Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet

Authors - Ebru S. Selen
View Article
mouseWB

EMBO Mol Med

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy

Authors - Nneka Southwell
View Article
humanIF

Hepatol Commun

Dysregulation of lipid metabolism in the pseudolobule promotes region-specific autophagy in hepatitis B liver cirrhosis

Authors - Wenjun Pu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Lana (Verified Customer) (06-21-2019)

Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissues, human and mouse, rat cell line.
ACOT2 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human and mouse, rat cell line. (Cat no:15633-1-AP)