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ACOT2 Polyclonal antibody
ACOT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15633-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, human kidney tissue, human testis tissue, mouse kidney tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue, human skeletal muscle tissue, human heart tissue, human testis tissue, human liver tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
15633-1-AP targets ACOT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACOT2 fusion protein Ag8093 |
| Full Name | acyl-CoA thioesterase 2 |
| Calculated Molecular Weight | 483 aa, 53 kDa |
| Observed Molecular Weight | 46-53 kDa |
| GenBank Accession Number | BC006335 |
| Gene Symbol | ACOT2 |
| Gene ID (NCBI) | 10965 |
| RRID | AB_2221535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxidative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACOT2 antibody 15633-1-AP | Download protocol |
| IHC protocol for ACOT2 antibody 15633-1-AP | Download protocol |
| IF protocol for ACOT2 antibody 15633-1-AP | Download protocol |
| IP protocol for ACOT2 antibody 15633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Immunol YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. | ||
Life Sci Alliance High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo | ||
Biomed Res Int Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression. | ||
J Biol Chem Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet | ||
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy | ||
Hepatol Commun Dysregulation of lipid metabolism in the pseudolobule promotes region-specific autophagy in hepatitis B liver cirrhosis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lana (Verified Customer) (06-21-2019) | Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.
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