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  • KD/KO Validated

Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12406-1-AP

Synonyms

ACO1, ACO 1, EC:4.2.1.3, Citrate hydro-lyase, Aconitase1



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells, mouse liver tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12406-1-AP targets Aconitase 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aconitase 1 fusion protein Ag3083
Full Name aconitase 1, soluble
Calculated Molecular Weight 889 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC018103
Gene Symbol Aconitase 1
Gene ID (NCBI) 48
RRIDAB_10642942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

Protocols

Product Specific Protocols
WB protocol for Aconitase 1 antibody 12406-1-APDownload protocol
IF protocol for Aconitase 1 antibody 12406-1-APDownload protocol
IP protocol for Aconitase 1 antibody 12406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Asparagine starvation suppresses histone demethylation through iron depletion

Authors - Jie Jiang
  • KD Validated
mouseWB

Biochim Biophys Acta Mol Basis Dis

Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.

Authors - Haipeng Cheng
human,mouseWB,IHC

Cell Biosci

Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.

Authors - Li Niu
humanWB

Drug Des Devel Ther

Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.

Authors - Chunguang Yang
humanWB

Biomolecules

Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Authors - Sara Ceccacci
humanWB

JOJ Ophthalmol

Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.

Authors - Lalit Pukhrambam Singh