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ACBD5 Polyclonal antibody

ACBD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21080-1-AP

Synonyms

ACBD5, KIAA1996

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ACBD5 Polyclonal antibody (21080-1-AP)

    WB analysis using 21080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ACBD5 Polyclonal antibody (21080-1-AP)

    WB analysis of mouse skeletal muscle using 21080-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, L02 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21080-1-AP targets ACBD5 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACBD5 fusion protein Ag15287
Full Name acyl-Coenzyme A binding domain containing 5
Calculated Molecular Weight 534 aa, 60 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC030555
Gene Symbol ACBD5
Gene ID (NCBI) 91452
RRIDAB_2878808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACBD5, also known as RDLKD. It is expected to be located in the peroxisome membrane, which is ubiquitinated in brain and kidney tissue. This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. The molecular weight of ACBD5 is 60 kDa.

Protocols

Product Specific Protocols
WB protocol for ACBD5 antibody 21080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

Authors - Pamela A Young
View Article
mouseWB

Mol Cell Biochem

Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina.

Authors - Yannick Das
View Article