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ACAT2 Polyclonal antibody

ACAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14755-1-AP

Synonyms

EC:2.3.1.9, Cytosolic acetoacetyl-CoA thiolase, ACTL, Acetyl-CoA transferase-like protein, Acetyl-CoA acetyltransferase, cytosolic



Tested Applications

Positive WB detected inCaco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14755-1-AP targets ACAT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT2 fusion protein Ag6503
Full Name acetyl-Coenzyme A acetyltransferase 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC000408
Gene Symbol ACAT2
Gene ID (NCBI) 39
RRIDAB_2220236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACAT2 antibody 14755-1-APDownload protocol
IHC protocol for ACAT2 antibody 14755-1-APDownload protocol
IF protocol for ACAT2 antibody 14755-1-APDownload protocol
IP protocol for ACAT2 antibody 14755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Res

Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.

Authors - Yanjing Guo
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
humanIHC

Cancer Cell Int

Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer

Authors - Enkui Zhang
mouseWB

iScience

Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.

Authors - George D Dalton
mouseWB

J Agric Food Chem

Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.

Authors - Yunxia Yang
humanWB

Food Res Int

Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.

Authors - Suwen Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Sarah-Eve (Verified Customer) (03-01-2023)

Very good working on PVDF membrane, blocking solution milk 5%

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human pulmonary artery smooth muscle cells
ACAT2 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary artery smooth muscle cells (Cat no:14755-1-AP)