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ACAT2 Polyclonal antibody
ACAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 14755-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Caco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue, human lung tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IHC | See 3 publications below |
Product Information
14755-1-AP targets ACAT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACAT2 fusion protein Ag6503 |
Full Name | acetyl-Coenzyme A acetyltransferase 2 |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 40-42 kDa |
GenBank Accession Number | BC000408 |
Gene Symbol | ACAT2 |
Gene ID (NCBI) | 39 |
RRID | AB_2220236 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ACAT2 antibody 14755-1-AP | Download protocol |
IHC protocol for ACAT2 antibody 14755-1-AP | Download protocol |
IF protocol for ACAT2 antibody 14755-1-AP | Download protocol |
IP protocol for ACAT2 antibody 14755-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Cancer Cell Int Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer | ||
iScience Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice. | ||
J Agric Food Chem Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice. | ||
Food Res Int Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah-Eve (Verified Customer) (03-01-2023) | Very good working on PVDF membrane, blocking solution milk 5%
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