Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 13880-1-AP

Synonyms

ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN



Tested Applications

Positive IHC detected inhuman lung tissue, human cartilage tissue, human lung cancer tissue, mouse lung tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inATDC-5 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13880-1-AP targets Aggrecan in IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aggrecan fusion protein Ag4985
Full Name aggrecan
Calculated Molecular Weight 250 kDa
GenBank Accession NumberBC036445
Gene Symbol ACAN
Gene ID (NCBI) 176
RRIDAB_2722780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Protocols

Product Specific Protocols
IHC protocol for Aggrecan antibody 13880-1-APDownload protocol
FC protocol for Aggrecan antibody 13880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
human

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
human

J Extracell Vesicles

miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.

Authors - Hongxing Hu
ratIF

Biomaterials

Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration

Authors - Lei Liu
mouse,ratIF

Cell Death Differ

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation

Authors - Guangjin Chen
mouse

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi