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- KD/KO Validated
ACADS Polyclonal antibody
ACADS Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 16623-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, COS-7 cells, mouse liver tissue, rat kidney tissue, rat liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 17 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
Product Information
The immunogen of 16623-1-AP is ACADS Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACADS fusion protein Ag9922 |
| Full Name | acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain |
| Calculated Molecular Weight | 412 aa, 44 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC025963 |
| Gene Symbol | ACADS |
| Gene ID (NCBI) | 35 |
| RRID | AB_10666165 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACADS antibody 16623-1-AP | Download protocol |
| IHC protocol for ACADS antibody 16623-1-AP | Download protocol |
| IP protocol for ACADS antibody 16623-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
EMBO Rep Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1. | ||
Cell Rep HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression. | ||
Aging (Albany NY) ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.
| ||
