ACADL Polyclonal antibody
ACADL Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17442-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, HepG2 cells, rat kidney tissue, HeLa cells, HEK-293 cells, NIH/3T3 cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
17442-1-AP targets ACADL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACADL fusion protein Ag11530 |
| Full Name | acyl-Coenzyme A dehydrogenase, long chain |
| Calculated Molecular Weight | 430 aa, 48 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC039063 |
| Gene Symbol | ACADL |
| Gene ID (NCBI) | 33 |
| RRID | AB_2219662 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACADL antibody 17442-1-AP | Download protocol |
| IHC protocol for ACADL antibody 17442-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients. | ||
Front Pharmacol Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease | ||
Front Pharmacol The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury | ||
Diabetologia Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation | ||
Cancer Metab A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells |
