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  • KD/KO Validated

ACC1 Polyclonal antibody

ACC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 21923-1-AP

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21923-1-AP targets ACC1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, pig, chicken, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACC1 fusion protein Ag16452
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated Molecular Weight 2383 aa, 275 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC137287
Gene Symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
RRIDAB_11042445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protocols

Product Specific Protocols
WB protocol for ACC1 antibody 21923-1-APDownload protocol
IHC protocol for ACC1 antibody 21923-1-APDownload protocol
IF protocol for ACC1 antibody 21923-1-APDownload protocol
IP protocol for ACC1 antibody 21923-1-APDownload protocol
FC protocol for ACC1 antibody 21923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
humanWB

Hepatology

Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.

Authors - Ming-Da Wang
  • KD Validated
humanWB

EMBO J

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Authors - Qichao Huang
mouseWB,IF

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang
  • KD Validated
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kishor (Verified Customer) (02-22-2019)

I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)