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  • Featured Product
  • KD/KO Validated

ABLIM1 Polyclonal antibody

ABLIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15129-1-AP

Synonyms

Actin-binding LIM protein family member 1, Actin-binding LIM protein 1, Actin-binding double zinc finger protein, actin binding LIM protein 1, abLIM-1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ABLIM1 Polyclonal antibody (15129-1-AP)

    WB analysis of HeLa using 15129-1-AP

    WB result of ABLIM1 antibody (15129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABLIM1 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using ABLIM1 Polyclonal antibody (15129-1-AP)

    WB analysis of HEK-293 using 15129-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using ABLIM1 Polyclonal antibody (15129-1-AP)

    WB analysis of NCI-H1299 using 15129-1-AP

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ABLIM1 Polyclonal antibody (15129-1-AP)

    WB analysis of HeLa using 15129-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ABLIM1 Polyclonal antibody (15129-1-AP)

    IHC staining of human liver using 15129-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ABLIM1 Polyclonal antibody (15129-1-AP)

    IHC staining of human liver using 15129-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ABLIM1 Polyclonal antibody (15129-1-AP)

    IF Staining of mouse skeletal muscle using 15129-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ABLIM1 Polyclonal antibody (15129-1-AP)

    IF Staining of mouse skeletal muscle using 15129-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ABLIM1 Polyclonal antibody (15129-1-AP)

    IF Staining of HEK-293 using 15129-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NCI-H1299 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15129-1-AP targets ABLIM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABLIM1 fusion protein Ag7258
Full Name actin binding LIM protein 1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 48-52 kDa, 105-110 kDa
GenBank Accession NumberBC002448
Gene Symbol ABLIM1
Gene ID (NCBI) 3983
RRIDAB_2257767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Due to the alternative splicing, ABLIM1 has multiple isoforms with predicted masses of 52, 70, and 88 kDa, respectively(PMID: 9245787). The phosphorylation or binding with SUMO2 may result in higher MW.

Protocols

Product Specific Protocols
WB protocol for ABLIM1 antibody 15129-1-APDownload protocol
IHC protocol for ABLIM1 antibody 15129-1-APDownload protocol
IF protocol for ABLIM1 antibody 15129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
  • KD Validated
View Article
mouseWB

J Cell Physiol

Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.

Authors - Haruna Narahara
  • KD Validated
View Article
humanWB

Int J Biol Sci

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Authors - Xin Dong
  • KD Validated
View Article
WB

Mol Biol Cell

Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Authors - Joleen S Cheah
View Article
humanIF

medRxiv

Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies

Authors - Young Me Yoon
View Article
mouseWB,IF

Circ Res

GSK-3β Localizes to the Cardiac Z-Disc to Maintain Length Dependent Activation

Authors - Marisa J Stachowski-Doll
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Joleen (Verified Customer) (05-29-2019)

ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MDCK GII
ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)