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  • Featured Product
  • KD/KO Validated

ABHD6 Polyclonal antibody

ABHD6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20494-1-AP

Synonyms

EC:3.1.1.23, Abhydrolase domain-containing protein 6, ABHD 6, 2-arachidonoylglycerol hydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABHD6 Polyclonal antibody (20494-1-AP)

    WB analysis using 20494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20494-1-AP (ABHD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ABHD6 Polyclonal antibody (20494-1-AP)

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using ABHD6 Polyclonal antibody (20494-1-AP)

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat spleen tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20494-1-AP targets ABHD6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD6 fusion protein Ag13968
Full Name abhydrolase domain containing 6
Calculated Molecular Weight 337 aa, 38 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC001698
Gene Symbol ABHD6
Gene ID (NCBI) 57406
RRIDAB_2878694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

α/β-hydrolase domain 6 (ABHD6) is a lipase linked to physiological functions affecting energy metabolism. ABHD6, which was first identified in the brain ( PMID: 18096503) , is a ubiquitously expressed lipase.

Protocols

Product Specific Protocols
WB protocol for ABHD6 antibody 20494-1-APDownload protocol
IHC protocol for ABHD6 antibody 20494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.

Authors - Jochen Schwenk
View Article
mouseWB

Pharmacol Res

Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice

Authors - Zhiwei Xue
View Article
humanWB

Mol Carcinog

ABHD6 suppresses colorectal cancer progression via AKT signaling pathway

Authors - Xiaoyu Xiong
  • KD Validated
View Article