• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ABHD15 Polyclonal antibody

ABHD15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22526-1-AP

Synonyms

ABHD15, Abhydrolase domain-containing protein 15, Alpha/beta hydrolase domain-containing protein 15, Protein ABHD15

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse lung tissue using ABHD15 Polyclonal antibody (22526-1-AP)

    WB analysis of mouse lung using 22526-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22526-1-AP (ABHD15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using ABHD15 Polyclonal antibody (22526-1-AP)

    WB analysis of Transfected HEK-293 using 22526-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22526-1-AP (ABHD15 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse lung tissue, Transfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22526-1-AP is ABHD15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD15 fusion protein Ag18334
Full Name abhydrolase domain containing 15
Calculated Molecular Weight 468 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC012476
Gene Symbol ABHD15
Gene ID (NCBI) 116236
RRIDAB_2879117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABHD15 belongs to the α/β-hydrolase superfamily which consists of various lipases, esterases, and proteases that share an α/β fold as a common structural feature. ABHD15 is highly expressed in adipose tissues and is required for the anti-lipolytic action of INS in white adipose tissue (WAT). ABHD15 expression is decreased in humans with obesity and diabetes compared to humans with obesity and normal glucose tolerance, identifying ABHD15 as a potential therapeutic target to mitigate INS resistance (PMID: 29768196).

Protocols

Product Specific Protocols
WB protocol for ABHD15 antibody 22526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

PMID: 29768196

Authors - Wenmin Xia
  • KO Validated
View Article
mouseWB

Mol Metab

ABHD15 regulates adipose tissue lipolysis and hepatic lipid accumulation.

Authors - Jacqueline Stöckli
  • KO Validated
View Article