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ABHD14B Polyclonal antibody

ABHD14B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20952-1-AP

Synonyms

Putative protein-lysine deacylase ABHD14B, EC:2.3.1.-, CIB, CCG1-interacting factor B, Alpha/beta hydrolase domain-containing protein 14B

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ABHD14B Polyclonal antibody (20952-1-AP)

    WB analysis of L02 using 20952-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using ABHD14B Polyclonal antibody (20952-1-AP)

    WB analysis of mouse small intestine using 20952-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ABHD14B Polyclonal antibody (20952-1-AP)

    IHC staining of human prostate hyperplasia using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ABHD14B Polyclonal antibody (20952-1-AP)

    IHC staining of human prostate hyperplasia using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using ABHD14B Polyclonal antibody (20952-1-AP)

    IHC staining of human spleen using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using ABHD14B Polyclonal antibody (20952-1-AP)

    IHC staining of human spleen using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ABHD14B Polyclonal antibody (20952-1-AP)

    IF Staining of HepG2 using 20952-1-AP

    Immunofluorescent analysis of HepG2 cells, using ABHD14B antibody 20952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inL02 cells, mouse small intestine tissue
Positive IHC detected inhuman prostate hyperplasia tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20952-1-AP targets ABHD14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD14B fusion protein Ag15097
Full Name abhydrolase domain containing 14B
Calculated Molecular Weight 210 aa, 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC007234
Gene Symbol ABHD14B
Gene ID (NCBI) 84836
RRIDAB_10733758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABHD14B antibody 20952-1-APDownload protocol
IHC protocol for ABHD14B antibody 20952-1-APDownload protocol
IF protocol for ABHD14B antibody 20952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols