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ABCG4 Polyclonal antibody

ABCG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14269-1-AP

Synonyms

ABCG4, WHITE2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of U-937 cells using ABCG4 Polyclonal antibody (14269-1-AP)

    WB analysis of U-937 using 14269-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ABCG4 Polyclonal antibody (14269-1-AP)

    WB analysis of mouse brain using 14269-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using ABCG4 Polyclonal antibody (14269-1-AP)

    WB analysis of U-937 using 14269-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ABCG4 Polyclonal antibody (14269-1-AP)

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ABCG4 Polyclonal antibody (14269-1-AP)

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inU-937 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14269-1-AP targets ABCG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCG4 fusion protein Ag5587
Full Name ATP-binding cassette, sub-family G (WHITE), member 4
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 60 kDa, 90-100 kDa
GenBank Accession NumberBC041091
Gene Symbol ABCG4
Gene ID (NCBI) 64137
RRIDAB_2220436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCG4 antibody 14269-1-APDownload protocol
IHC protocol for ABCG4 antibody 14269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

PLoS One

High ABCG4 Expression Is Associated with Poor Prognosis in Non-Small-Cell Lung Cancer Patients Treated with Cisplatin-Based Chemotherapy.

Authors - Guang Yang
View Article