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ABCF2 Polyclonal antibody

ABCF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10226-1-AP

Synonyms

M ABC1, Iron-inhibited ABC transporter 2, HUSSY-18, HUSSY18, ATP-binding cassette sub-family F member 2

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using ABCF2 Polyclonal antibody (10226-1-AP)

    WB analysis of NIH/3T3 using 10226-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10226-1-AP (ABCF2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ABCF2 Polyclonal antibody (10226-1-AP)

    WB analysis of HEK-293 using 10226-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10226-1-AP (ABCF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ABCF2 Polyclonal antibody (10226-1-AP)

    IP experiment of HeLa using 10226-1-AP

    IP result of anti-ABCF2 (IP:10226-1-AP, 4ug; Detection:10226-1-AP 1:300) with HeLa cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ABCF2 Polyclonal antibody (10226-1-AP)

    IHC staining of human colon cancer using 10226-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10226-1-AP (ABCF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCF2 Polyclonal antibody (10226-1-AP)

    IF Staining of HeLa using 10226-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10226-1-AP (ABCF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10226-1-AP targets ABCF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCF2 fusion protein Ag0310
Full Name ATP-binding cassette, sub-family F (GCN20), member 2
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC001661
Gene Symbol ABCF2
Gene ID (NCBI) 10061
RRIDAB_2304888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCF2 (ABC28, M-ABC1, HUSSY-18, EST133090, DKFZp586K1823) is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins are composed of transmembrane domains (TMDs), and nucleotide binding domains (NBDs, or ATP-binding cassettes, ABSs). Based on their sequence similarity scores, the members of the human ABC protein family can be grouped into eight subfamilies, including the MDR/TAP, the ALD, the MRP/CFTR, the ABC1, the White, the RNAseL inhibitor, the ANSA, and the GCN20 subfamilies. ABCF2 is a member of the GCN20 subfamily. ABC proteins transport various molecules across extra- and intracellular membranes.

Protocols

Product Specific Protocols
WB protocol for ABCF2 antibody 10226-1-APDownload protocol
IHC protocol for ABCF2 antibody 10226-1-APDownload protocol
IF protocol for ABCF2 antibody 10226-1-APDownload protocol
IP protocol for ABCF2 antibody 10226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta
View Article