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- KD/KO Validated
ABCF1 Polyclonal antibody
ABCF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13950-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, HepG2 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13950-1-AP targets ABCF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABCF1 fusion protein Ag5061 |
| Full Name | ATP-binding cassette, sub-family F (GCN20), member 1 |
| Calculated Molecular Weight | 92 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC034488 |
| Gene Symbol | ABCF1 |
| Gene ID (NCBI) | 23 |
| RRID | AB_2242044 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCF1 antibody 13950-1-AP | Download protocol |
| IHC protocol for ABCF1 antibody 13950-1-AP | Download protocol |
| IF protocol for ABCF1 antibody 13950-1-AP | Download protocol |
| IP protocol for ABCF1 antibody 13950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunity The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock. | ||
Sci Adv ATP-binding cassette protein ABCF1 couples transcription and genome surveillance in embryonic stem cells through low-complexity domain.
| ||
Proteomics Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma |
