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ABCD1 Monoclonal antibody

ABCD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H8E5

Cat no : 60153-1-Ig

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ABCD1 Monoclonal antibody (60153-1-Ig)

    WB analysis of HeLa using 60153-1-Ig

    WB result of ABCD1 antibody (60153-1-Ig, 1:1000) with si-Control and si-ABCD1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Monoclonal antibody (60153-1-Ig)

    WB analysis of HeLa using 60153-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60153-1-Ig (ABCD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCD1 Monoclonal antibody (60153-1-Ig)

    IF Staining of HeLa using 60153-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCD1 antibody (60153-1-Ig, Clone: 1H8E5 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).DAPI (blue).

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60153-1-Ig targets ABCD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ABCD1 fusion protein Ag12757
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated Molecular Weight 745 aa, 83 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC025358
Gene Symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_10640436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Protocols

Product Specific Protocols
WB protocol for ABCD1 antibody 60153-1-IgDownload protocol
IF protocol for ABCD1 antibody 60153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols