human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11159-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11159-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 11159-1-AP
IP result of anti-ABCD1 (IP:11159-1-AP, 4ug; Detection:11159-1-AP 1:500) with mouse liver tissue lysate 5000ug.
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11159-1-AP
Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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human brain tissue, HeLa cells, mouse liver tissue
Positive IP detected in
mouse liver tissue
Positive IHC detected in
human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.
human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11159-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11159-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung cancer using 11159-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of mouse liver using 11159-1-AP
IP result of anti-ABCD1 (IP:11159-1-AP, 4ug; Detection:11159-1-AP 1:500) with mouse liver tissue lysate 5000ug.
IF/ICC Figures
IF Staining of HeLa using 11159-1-AP
Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ABCD1 Polyclonal antibody
Catalog Number
11159-1-AP
Citations
2
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
