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  • Featured Product
  • KD/KO Validated

ABCB10 Polyclonal antibody

ABCB10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14628-1-AP

Synonyms

ABC transporter 10 protein, ABCB10, ABC-me, EST20237, M ABC2, MTABC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCB10 Polyclonal antibody (14628-1-AP)

    WB analysis using 14628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14628-1-AP (ABCB10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using ABCB10 Polyclonal antibody (14628-1-AP)

    IP experiment of K-562 using 14628-1-AP

    IP result of anti-ABCB10 (IP:14628-1-AP, 4ug; Detection:14628-1-AP 1:300) with K-562 cells lysate 3200ug.

Tested Applications

Positive WB detected inJurkat cells, K-562 cellls
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14628-1-AP targets ABCB10 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB10 fusion protein Ag6203
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 10
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC064930
Gene Symbol ABCB10
Gene ID (NCBI) 23456
RRIDAB_2220668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCB10 antibody 14628-1-APDownload protocol
IP protocol for ABCB10 antibody 14628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

ABCB10 depletion reduces unfolded protein response in mitochondria.

Authors - Masato Yano
  • KD Validated
View Article
humanWB

PeerJ

TMEM65 is a mitochondrial inner-membrane protein.

Authors - Naotaka Nishimura
View Article
mouseFC

Exp Hematol

Abcb10 regulates murine hematopoietic stem cell potential and erythroid differentiation

Authors - Ayano Yahagi
View Article

Mol Cell

Unraveling ETC complex I function in ferroptosis reveals a potential ferroptosis-inducing therapeutic strategy for LKB1-deficient cancers

Authors - Chao Mao
View Article