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  • Featured Product
  • KD/KO Validated

ABCA7 Polyclonal antibody

ABCA7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25339-1-AP

Synonyms

ABCA SSN, ABCA7, ABCX, Autoantigen SS N, Macrophage ABC transporter

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ABCA7 Polyclonal antibody (25339-1-AP)

    WB analysis of mouse brain using 25339-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25339-1-AP (ABCA7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ABCA7 Polyclonal antibody (25339-1-AP)

    WB analysis of mouse brain using 25339-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25339-1-AP (ABCA7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ABCA7 Polyclonal antibody (25339-1-AP)

    IHC staining of rat brain using 25339-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25339-1-AP (ABCA7 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using ABCA7 Polyclonal antibody (25339-1-AP)

    IHC staining of rat brain using 25339-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25339-1-AP (ABCA7 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25339-1-AP targets ABCA7 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATP-binding cassette, sub-family A (ABC1), member 7
Calculated Molecular Weight 234 kDa
Observed Molecular Weight 200-240 kDa
GenBank Accession NumberNM_019112
Gene Symbol ABCA7
Gene ID (NCBI) 10347
RRIDAB_2880035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCA7 antibody 25339-1-APDownload protocol
IHC protocol for ABCA7 antibody 25339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Alzheimers Dis

ABCA7 Downregulation Modifies Cellular Cholesterol Homeostasis and Decreases Amyloid-β Peptide Efflux in an in vitro Model of the Blood-Brain Barrier.

Authors - Yordenca Lamartinière
View Article
humanWB

Oncol Lett

ATP-binding cassette transporter A7 accelerates epithelial-to-mesenchymal transition in ovarian cancer cells by upregulating the transforming growth factor-β signaling pathway.

Authors - Xia Liu
  • KD Validated
View Article
mouseWB

Mol Psychiatry

ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism

Authors - Keiji Kawatani
View Article