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ABCA2 Polyclonal antibody

ABCA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20681-1-AP

Synonyms

ABC transporter ABCA2, ABC2, ABCA2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using ABCA2 Polyclonal antibody (20681-1-AP)

    WB analysis of mouse thymus using 20681-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ABCA2 Polyclonal antibody (20681-1-AP)

    WB analysis of L02 using 20681-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ABCA2 Polyclonal antibody (20681-1-AP)

    WB analysis of HepG2 using 20681-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ABCA2 Polyclonal antibody (20681-1-AP)

    IHC staining of mouse brain using 20681-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20681-1-AP (ABCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ABCA2 Polyclonal antibody (20681-1-AP)

    IF Staining of HepG2 using 20681-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20681-1-AP (ABCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse thymus tissue, HepG2 cells, L02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20681-1-AP targets ABCA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATP-binding cassette, sub-family A (ABC1), member 2
Calculated Molecular Weight 270 kDa
Observed Molecular Weight 270 kDa
GenBank Accession NumberNM_001606
Gene Symbol ABCA2
Gene ID (NCBI) 20
RRIDAB_10859780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCA2, also named as ABC2 and KIAA1062, belongs to the ABC transporter ABCA2, a member of the ATP-binding cassette (ABC) transporter family, is primarily expressed in brain and is involved in brain sterol homeostasis and associated with early onset Alzheimer's disease. ABCA2 is mainly localized in endosomes and lysosomes of oligodendrocytes and Schwann cells. It has also be reported as a marker of neural progenitor in the adult rodent brain. (PMID: 16539677)

Protocols

Product Specific Protocols
WB protocol for ABCA2 antibody 20681-1-APDownload protocol
IHC protocol for ABCA2 antibody 20681-1-APDownload protocol
IF protocol for ABCA2 antibody 20681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols