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AATF Polyclonal antibody

AATF Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10282-1-AP

Synonyms

Protein AATF, HSPC277, DED, CHE1, CHE 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AATF Polyclonal antibody (10282-1-AP)

    WB analysis using 10282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AATF Polyclonal antibody (10282-1-AP)

    WB analysis of HepG2 using 10282-1-AP

    WB result of AATF antibody (10282-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells.

  • Western Blot (WB) analysis of mouse brain tissue using AATF Polyclonal antibody (10282-1-AP)

    WB analysis of mouse brain using 10282-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AATF Polyclonal antibody (10282-1-AP)

    IF Staining of HeLa using 10282-1-AP

    Immunofluorescent analysis of Hela cells, using AATF antibody 10282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293T cells, mouse brain tissue, HepG2 cells, HeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10282-1-AP targets AATF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AATF fusion protein Ag0195
Full Name apoptosis antagonizing transcription factor
Calculated Molecular Weight 63 kDa
Observed Molecular Weight80-95 kDa
GenBank Accession NumberBC000591
Gene Symbol AATF
Gene ID (NCBI) 26574
RRIDAB_2219887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

Protocols

Product Specific Protocols
WB protocol for AATF antibody 10282-1-APDownload protocol
IF protocol for AATF antibody 10282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols